Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144743_hpu8 CAGATGACAGCTTACACCAATCAGGCTGATTG(+AGGACA)GTACCAAAAAGCACACATTTCTGCTTTTATT NGG 10 8 17 8 24 0 100 5 10
    240613144741_p1rp AATAAAATAAAAGCAGAAATGTGTGCTTTTTG(+AGGACA)GTACCAATCAGCCTGATTGGTGTAAGCTGTC NGG 10 8 17 8 24 0 100 5 10
    240613144738_2jby GAGCCTCCGCGGAGCCGTCCGTCTGTCCCTGG(+AGGACA)GATCCTCGAGCGTCTGGCGGTTCCCTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613144737_zymw TGTGGTGCTGAGGGAACCGCCAGACGCTCGAG(+AGGACA)GATCCCAGGGACAGACGGACGGCTCCGCGGA NGG 10 8 17 8 24 0 100 5 10
    240613144736_ufwm TCATGACCCATCTTCAGAGACTGACATTTCAG(+AGGACA)GATCCATATTAAAGCATTCATTTTCTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613144735_8xx7 AATGTAACAAAGAAAATGAATGCTTTAATATG(+AGGACA)GATCCTGAAATGTCAGTCTCTGAAGATGGGT NGG 10 8 17 8 24 0 100 5 10
    240613144733_j627 TATAATCCTAAATCAGTATCTGTATGGAATGG(+AGGACA)GTACCTTGCCCTGCAGTTTCCATGAGGAATT NGG 10 8 17 8 24 0 100 5 10
    240613144732_4afp TGTAGAATTCCTCATGGAAACTGCAGGGCAAG(+AGGACA)GTACCCATTCCATACAGATACTGATTTAGGA NGG 10 8 17 8 24 0 100 5 10
    240613144730_37mo CCTTATATCTACGGAGCTGACAATATCTTATG(+AGGACA)GATCCTGCAAGGCCTTGTGGGAAGGTGATGT NGG 10 8 17 8 24 0 100 5 10
    240613144729_vcvk ATAGCACATCACCTTCCCACAAGGCCTTGCAG(+AGGACA)GATCCATAAGATATTGTCAGCTCCGTAGATA NGG 10 8 17 8 24 0 100 5 10