Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144757_05fa AATAAAAGCAGTAAATGCCTGTTAGTAGCAAG(+AGGACA)GATCCTCAGTAAAAGTGAGCGGGTGGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613144755_i4u6 GGCTACAGAGCCACCCGCTCACTTTTACTGAG(+AGGACA)GATCCTTGCTACTAACAGGCATTTACTGCTT NGG 10 8 17 8 24 0 100 5 10
    240613144754_f5in TCAATGAAATCTCACATGCTCATTTTACTCAG(+AGGACA)GATCCAGTAAGAATGCAAAGGGGTTGCTAAG NGG 10 8 17 8 24 0 100 5 10
    240613144753_kal5 TTTACCTTAGCAACCCCTTTGCATTCTTACTG(+AGGACA)GATCCTGAGTAAAATGAGCATGTGAGATTTC NGG 10 8 17 8 24 0 100 5 10
    240613144752_6nt7 CAAACAATTGTGAGCTGCCATGTAGATGCTAG(+AGGACA)GATCCCTGGTCCTCTAGAAGAGCCATCTCTC NGG 10 8 17 8 24 0 100 5 10
    240613144749_unye GGCTAGAGAGATGGCTCTTCTAGAGGACCAGG(+AGGACA)GATCCTAGCATCTACATGGCAGCTCACAATT NGG 10 8 17 8 24 0 100 5 10
    240613144748_98gl CCTGCCCCATCCTAGCACCCCCAGAGATTTAG(+AGGACA)GTACCAAGAGTAAGGCAGCCCAAGCCAATAT NGG 10 8 17 8 24 0 100 5 10
    240613144747_6wca GTCACATATTGGCTTGGGCTGCCTTACTCTTG(+AGGACA)GTACCTAAATCTCTGGGGGTGCTAGGATGGG NGG 10 8 17 8 24 0 100 5 10
    240613144745_ga38 TGGCCTAGACCTTTATATGCTGATCAGGTTGG(+AGGACA)GTACCAACTGTCAGCCTGTCTGCTGTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240613144744_jrtr GAGATGGGAGACAGCAGACAGGCTGACAGTTG(+AGGACA)GTACCCAACCTGATCAGCATATAAAGGTCTA NGG 10 8 17 8 24 0 100 5 10