Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144834_3qv5 GCTGGTACAGACTCCTATGGAGTTTTGCTGAG(+AGGACA)GTACCAACCATGGGAGAGCACAAGATGTTTG NGG 10 8 17 8 24 0 100 5 10
    240613144832_c72q CTCTCCAAACATCTTGTGCTCTCCCATGGTTG(+AGGACA)GTACCTCAGCAAAACTCCATAGGAGTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240613144831_ad79 GAGGATGCTCGGAGTGGCACCAGAGATGGCAG(+AGGACA)GTACCACCCTGACTCTAGTTGTCTGTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613144829_an8k GTGAGCAGCAACAGACAACTAGAGTCAGGGTG(+AGGACA)GTACCTGCCATCTCTGGTGCCACTCCGAGCA NGG 10 8 17 8 24 0 100 5 10
    240613144827_w7a0 AGTGTGCTGTTGCCTATCTTTTTGACATCTGG(+AGGACA)GTACCTGTGATGGCCTACAGGAAGCCCACAC NGG 10 8 17 8 24 0 100 5 10
    240613144826_yv8a ATTTTGTGTGGGCTTCCTGTAGGCCATCACAG(+AGGACA)GTACCCAGATGTCAAAAAGATAGGCAACAGC NGG 10 8 17 8 24 0 100 5 10
    240613144825_0kto GTGGTAGGTTTTCATGGTTATGACTACATGTG(+AGGACA)GTACCAGTTCTGTGTTATTTGTTTCCTGGTC NGG 10 8 17 8 24 0 100 5 10
    240613144824_wslu TGGTAGACCAGGAAACAAATAACACAGAACTG(+AGGACA)GTACCACATGTAGTCATAACCATGAAAACCT NGG 10 8 17 8 24 0 100 5 10
    240613144822_gtbr TACTTTGCTGTATTCAAGTCTGTTTCTCAGTG(+AGGACA)GATCCAGTGAACTAAATTCTAACATTTGAAC NGG 10 8 17 8 24 0 100 5 10
    240613144821_d60c AAACTGTTCAAATGTTAGAATTTAGTTCACTG(+AGGACA)GATCCACTGAGAAACAGACTTGAATACAGCA NGG 10 8 17 8 24 0 100 5 10