Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144727_pk9j CAGGGAAATTGCTACAGATGTTTCACAACCTG(+AGGACA)GATCCATCCTGTCAGTCAAACAGCAACAACC NGG 10 8 17 8 24 0 100 5 10
    240613144726_tozb GAAATGGTTGTTGCTGTTTGACTGACAGGATG(+AGGACA)GATCCAGGTTGTGAAACATCTGTAGCAATTT NGG 10 8 17 8 24 0 100 5 10
    240613144724_tpm7 ACTTAAGTTCACACCAGGAGTTAGGCGCTCTG(+AGGACA)GATCCTGAGCCAGGCAGGCACAAAGGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613144723_1ian CTCTAAGGAACCTTTGTGCCTGCCTGGCTCAG(+AGGACA)GATCCAGAGCGCCTAACTCCTGGTGTGAACT NGG 10 8 17 8 24 0 100 5 10
    240613144722_k2cj TCAAATCCTGCAGTAGTGAGTAGCTATGAATG(+AGGACA)GATCCTATTAACCGGGTGGGAGGGGGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613144720_oo1k CTGTTTCCACCCCCCTCCCACCCGGTTAATAG(+AGGACA)GATCCATTCATAGCTACTCACTACTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613144718_3xxy GTGCCATCTCTGAATGCAGAACTCATGTCCAG(+AGGACA)GTACCAGTACTGTAGTGGAGCATAGGATACA NGG 10 8 17 8 24 0 100 5 10
    240613144717_naj3 TGTGCTGTATCCTATGCTCCACTACAGTACTG(+AGGACA)GTACCTGGACATGAGTTCTGCATTCAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613144716_msvt GTAATCTCTTCCCTCACATGTAACAAACTCTG(+AGGACA)GATCCAACCCTCAGTACTACGGATCTAAATC NGG 10 8 17 8 24 0 100 5 10
    240613144714_bb8p AGTGAGATTTAGATCCGTAGTACTGAGGGTTG(+AGGACA)GATCCAGAGTTTGTTACATGTGAGGGAAGAG NGG 10 8 17 8 24 0 100 5 10