Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144554_jzhf AGTGAGGGCTTTGTCCCAAGAAGGCTGTGTGG(+AGGACA)GTACCAGATTCTGCAAGTGTTGAAGTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240613144553_vpwc GAACTATAGAACTTCAACACTTGCAGAATCTG(+AGGACA)GTACCCACACAGCCTTCTTGGGACAAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240613144551_fcyp AGGTGGCCATGAGCACCTAATGTGGGTGCTGG(+AGGACA)GATCCTGAGTCAATGCCTCTGCAAGAGCCGT NGG 10 8 17 8 24 0 100 5 10
    240613144550_5jud GTGCGACGGCTCTTGCAGAGGCATTGACTCAG(+AGGACA)GATCCCAGCACCCACATTAGGTGCTCATGGC NGG 10 8 17 8 24 0 100 5 10
    240613144549_00at TCCCTTCCTGGATCACAATGTCTGGGGATTAG(+AGGACA)GATCCATCCATCATCCTAGCCAAGAACTGCA NGG 10 8 17 8 24 0 100 5 10
    240613144547_712u CTTCATGCAGTTCTTGGCTAGGATGATGGATG(+AGGACA)GATCCTAATCCCCAGACATTGTGATCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613144546_h1zw ACATTTGGAGCTCCCAATGCCTGTGTCGTTGG(+AGGACA)GATCCAGTCTGGGAACTAGGACACATTTGGC NGG 10 8 17 8 24 0 100 5 10
    240613144545_ouf9 TGTTTGCCAAATGTGTCCTAGTTCCCAGACTG(+AGGACA)GATCCCAACGACACAGGCATTGGGAGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613144542_24t0 CTTTAAGGTACAAGTGTAAAAACAAGTTCCGG(+AGGACA)GATCCAGTTTCTATCCGTGTGTGTTATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613144541_uejg GCTTTGACCATAACACACACGGATAGAAACTG(+AGGACA)GATCCCGGAACTTGTTTTTACACTTGTACCT NGG 10 8 17 8 24 0 100 5 10