Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144540_hjf1 CTCAGTTGTCGCGGTTTGGTTTCATCGTGATG(+AGGACA)GATCCGCTGTCGTGAGTAAGGTATTTATTGT NGG 10 8 17 8 24 0 100 5 10
    240613144539_s7l5 TTCATACAATAAATACCTTACTCACGACAGCG(+AGGACA)GATCCATCACGATGAAACCAAACCGCGACAA NGG 10 8 17 8 24 0 100 5 10
    240613144537_w0rx ATGTCAGACAGCACAGAAGGGAGAAGGAGGTG(+AGGACA)GATCCCCAAACCCACCTCCAGTGATCTACTT NGG 10 8 17 8 24 0 100 5 10
    240613144535_8euy GGAGGAAGTAGATCACTGGAGGTGGGTTTGGG(+AGGACA)GATCCACCTCCTTCTCCCTTCTGTGCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240613144534_3nmy GACATCAGAGGGAAACACTGAATAACTTTCTG(+AGGACA)GATCCTGATTGTTGGTCTATTCTGCAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240613144533_64my TTGATGTCACTGCAGAATAGACCAACAATCAG(+AGGACA)GATCCAGAAAGTTATTCAGTGTTTCCCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613144532_ikot AAAAAAAAGCTTGGAAAAGTGAATTTAAAGAG(+AGGACA)GATCCAAGGTAACATTCTTGAACTAAGATGC NGG 10 8 17 8 24 0 100 5 10
    240613144530_5mut GGAAGGCATCTTAGTTCAAGAATGTTACCTTG(+AGGACA)GATCCTCTTTAAATTCACTTTTCCAAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613144528_6wt4 TGTCTCAAAGCACAGAAGTCTCAAAACTGTGG(+AGGACA)GTACCTAGAGTCCCACAATGCCTCACTTCTT NGG 10 8 17 8 24 0 100 5 10
    240613144527_b1mx ATGAAAAGAAGTGAGGCATTGTGGGACTCTAG(+AGGACA)GTACCCACAGTTTTGAGACTTCTGTGCTTTG NGG 10 8 17 8 24 0 100 5 10