Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144438_g6ez AAAATAATATTTGGGTGAATGATACTGCCATG(+AGGACA)GATCCTTTTGTTTAATTTTATATATAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240613144437_es5q TATCCGAGCTTATATATAAAATTAAACAAAAG(+AGGACA)GATCCATGGCAGTATCATTCACCCAAATATT NGG 10 8 17 8 24 0 100 5 10
    240613144435_jh9r TCACAAGCAGGTGTTTATGTCTGAAATTGGAG(+AGGACA)GTACCCATTATCTGAAAATTCCTCTCCCATA NGG 10 8 17 8 24 0 100 5 10
    240613144434_n949 TACCTTATGGGAGAGGAATTTTCAGATAATGG(+AGGACA)GTACCTCCAATTTCAGACATAAACACCTGCT NGG 10 8 17 8 24 0 100 5 10
    240613144433_680o CACTTTAAAACACTGTGGAGCCAGGAATAATG(+AGGACA)GTACCCACCTGTAATCCCAGCACCTGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240613144431_5o4h TCCTGTCTCCCAGGTGCTGGGATTACAGGTGG(+AGGACA)GTACCATTATTCCTGGCTCCACAGTGTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613144429_pjsx CATGTCTCTGGATGATACCTAGGCATCTCTAG(+AGGACA)GTACCCAGAGATGGAGCCCTGGGAAGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240613144428_txxw GAACGTTCTTCTTCCCAGGGCTCCATCTCTGG(+AGGACA)GTACCTAGAGATGCCTAGGTATCATCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613144426_5s1j TGGCAAGAATTACATATTCTGTATTTTTTGTG(+AGGACA)GTACCAAATGCACTGCCTTGTACTTAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240613144425_16wp TAGCACTTGCTAAGTACAAGGCAGTGCATTTG(+AGGACA)GTACCACAAAAAATACAGAATATGTAATTCT NGG 10 8 17 8 24 0 100 5 10