Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144526_idvh CACAGCTCCTTAGTTGCTGGGCAGGGCTCAAG(+AGGACA)GATCCTTCCCCACTTCATGTGGGATGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613144524_i3zh GTCTGGAACTCATCCCACATGAAGTGGGGAAG(+AGGACA)GATCCTTGAGCCCTGCCCAGCAACTAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613144523_r1j7 CTGAACATTTAGAACTCAGAGCCCCATTCATG(+AGGACA)GTACCTACAGCCCATCTGGAAAATTCTTCAA NGG 10 8 17 8 24 0 100 5 10
    240613144522_axtk TACTATTGAAGAATTTTCCAGATGGGCTGTAG(+AGGACA)GTACCATGAATGGGGCTCTGAGTTCTAAATG NGG 10 8 17 8 24 0 100 5 10
    240613144520_x1d4 AGGCACCTTCCTGTAGAAGAGCCCATAGTCAG(+AGGACA)GTACCCAATTACACCTTGGTGTTGTCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240613144519_mwjg ATGCATCTTGGACAACACCAAGGTGTAATTGG(+AGGACA)GTACCTGACTATGGGCTCTTCTACAGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613144516_wmjz GTGAGTCTTGTTCATTGTGTAGAGAAACCCAG(+AGGACA)GTACCCAAAGTCGGCAGTCTTTCCCAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240613144515_m2vn ACATGGACCCTGGGAAAGACTGCCGACTTTGG(+AGGACA)GTACCTGGGTTTCTCTACACAATGAACAAGA NGG 10 8 17 8 24 0 100 5 10
    240613144513_g88g ACTGTGGGGCAAGGAAAGTGACACTGACCTTG(+AGGACA)GATCCACACTGCCTGGGGTTGTGTTAGTTAG NGG 10 8 17 8 24 0 100 5 10
    240613144511_c70g GTCAGCTAACTAACACAACCCCAGGCAGTGTG(+AGGACA)GATCCAAGGTCAGTGTCACTTTCCTTGCCCC NGG 10 8 17 8 24 0 100 5 10