Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144455_zrl3 TTTTCTGAGGGTGGGGGTGGGTACTAATTGAG(+AGGACA)GATCCTTCAAGACTGAAAATCTATTGTCTTA NGG 10 8 17 8 24 0 100 5 10
    240613144454_1sde TTATCTAAGACAATAGATTTTCAGTCTTGAAG(+AGGACA)GATCCTCAATTAGTACCCACCCCCACCCTCA NGG 10 8 17 8 24 0 100 5 10
    240613144452_1ctk CCTGCTCTGTCCTCTCACTTGATCAGAGCTGG(+AGGACA)GATCCCTGCTACTTCCCAGTGCCCAAGGGTT NGG 10 8 17 8 24 0 100 5 10
    240613144450_bhbj AGAGGAACCCTTGGGCACTGGGAAGTAGCAGG(+AGGACA)GATCCCAGCTCTGATCAAGTGAGAGGACAGA NGG 10 8 17 8 24 0 100 5 10
    240613144449_k8hb GATGTCCAGTCTTAAGGAATGAGCAATTACTG(+AGGACA)GATCCCTTACCTATCTGTCTACTGTTGGACT NGG 10 8 17 8 24 0 100 5 10
    240613144448_v600 CAGACAGTCCAACAGTAGACAGATAGGTAAGG(+AGGACA)GATCCAGTAATTGCTCATTCCTTAAGACTGG NGG 10 8 17 8 24 0 100 5 10
    240613144446_voxi CCTCTCGGGCTAGGAGGGCGCTGGATGGCGGG(+AGGACA)GTACCCTCCCTCCTGCACCGACTCCACTGAA NGG 10 8 17 8 24 0 100 5 10
    240613144444_o67p ACTACTTCAGTGGAGTCGGTGCAGGAGGGAGG(+AGGACA)GTACCCCGCCATCCAGCGCCCTCCTAGCCCG NGG 10 8 17 8 24 0 100 5 10
    240613144443_5hru ATACACAGTCCGGTGGGGGAGGGGCTGGGCTG(+AGGACA)GATCCTGGTAGGGGTAAGCGGTAGTGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613144440_q1bs CTGCAAGCTCCACTACCGCTTACCCCTACCAG(+AGGACA)GATCCAGCCCAGCCCCTCCCCCACCGGACTG NGG 10 8 17 8 24 0 100 5 10