Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144635_z54z TCTCTGGCTTAAAGGGTCCCAAGTAGGCCACG(+AGGACA)GTACCTTTCCTGAGACTTTCAAGGAGTTGAT NGG 10 8 17 8 24 0 100 5 10
    240613144634_a19s TACCCATCAACTCCTTGAAAGTCTCAGGAAAG(+AGGACA)GTACCGTGGCCTACTTGGGACCCTTTAAGCC NGG 10 8 17 8 24 0 100 5 10
    240613144632_zcmz TTCTGGGTCAAGGATGCATCCCTGACAACCAG(+AGGACA)GATCCCAGAACCTTTGCCTGCTGATCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240613144631_s9sg TTAGCTCTCAGATCAGCAGGCAAAGGTTCTGG(+AGGACA)GATCCTGGTTGTCAGGGATGCATCCTTGACC NGG 10 8 17 8 24 0 100 5 10
    240613144629_hc3q TCGGAAGGTTGTTCCATTCCTCCACAGCACTG(+AGGACA)GTACCTTAAAAGCAAACAAAAACCAACGAGA NGG 10 8 17 8 24 0 100 5 10
    240613144628_7oiy CATAGTCTCGTTGGTTTTTGTTTGCTTTTAAG(+AGGACA)GTACCAGTGCTGTGGAGGAATGGAACAACCT NGG 10 8 17 8 24 0 100 5 10
    240613144627_w033 TAGATGGTCAACTTGATCCTGATTCAACTTTG(+AGGACA)GTACCTGATATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240613144625_oqn1 GACAAATGGACAAATTTCTGGACAGATATCAG(+AGGACA)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240613144624_73wx AGTTTAACTTCTGGAGTCCATATAAAGAGCTG(+AGGACA)GATCCAAAGTGTGCATTTGCAATCCCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613144623_itv1 GTTCAGTGCTGGGATTGCAAATGCACACTTTG(+AGGACA)GATCCAGCTCTTTATATGGACTCCAGAAGTT NGG 10 8 17 8 24 0 100 5 10