Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144510_ni5w AAGAAGGGAGATAGACCTCAAACTAGTTCTTG(+AGGACA)GTACCTCACCTTCATCCCCCACCCACCCACT NGG 10 8 17 8 24 0 100 5 10
    240613144509_bseo TCGGGAGTGGGTGGGTGGGGGATGAAGGTGAG(+AGGACA)GTACCAAGAACTAGTTTGAGGTCTATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240613144507_6zgm GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+AGGACA)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613144505_rva1 GGATGGACCATCTAGTGATTGCCATATGCAGG(+AGGACA)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240613144504_bdq0 TTAGTCATCATGGATTGTCACTCCTTGAGAAG(+AGGACA)GATCCAGAGGTGTAGATTTGTTGGGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240613144502_ia40 GACATACTTCCCCCAACAAATCTACACCTCTG(+AGGACA)GATCCTTCTCAAGGAGTGACAATCCATGATG NGG 10 8 17 8 24 0 100 5 10
    240613144501_ljc6 GTGGGATTCTCTCTATGTGTGTCTTCTACATG(+AGGACA)GATCCAGAGAGCAGTTCCTAATGGAAACGAT NGG 10 8 17 8 24 0 100 5 10
    240613144459_5gt7 GATACATCGTTTCCATTAGGAACTGCTCTCTG(+AGGACA)GATCCATGTAGAAGACACACATAGAGAGAAT NGG 10 8 17 8 24 0 100 5 10
    240613144458_v79g GTTGCCCTTTGATGCTGAGATCTTCCGGTATG(+AGGACA)GATCCTCCGTGAAGGCAGGCTCAGGGTGAGA NGG 10 8 17 8 24 0 100 5 10
    240613144456_nrey TCTCATCTCACCCTGAGCCTGCCTTCACGGAG(+AGGACA)GATCCATACCGGAAGATCTCAGCATCAAAGG NGG 10 8 17 8 24 0 100 5 10