Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144920_uo4h AACGGCCTCAACAGGGCCGAGAGGCTGACAGG(+AGGACA)GTACCTGGAGTCCAGTTACTCACTGGCTGTC NGG 10 8 17 8 24 0 100 5 10
    240613144918_1s17 ACTCAGACAGCCAGTGAGTAACTGGACTCCAG(+AGGACA)GTACCCTGTCAGCCTCTCGGCCCTGTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240613144916_xbdp TCTACCCCATGTGGTCTGCCTCTGGTGTGGTG(+AGGACA)GTACCTGGTCCTTTTTATACCCCTTCCACCT NGG 10 8 17 8 24 0 100 5 10
    240613144914_tv55 AGCCAAGGTGGAAGGGGTATAAAAAGGACCAG(+AGGACA)GTACCACCACACCAGAGGCAGACCACATGGG NGG 10 8 17 8 24 0 100 5 10
    240613144912_lue3 CCTTCTGCACTCAGCCTTTCCTTGGGGCTAGG(+AGGACA)GATCCATCACATGGGGTAAGGCCTAGCCTAA NGG 10 8 17 8 24 0 100 5 10
    240613144911_0gyx TTGGATTAGGCTAGGCCTTACCCCATGTGATG(+AGGACA)GATCCCTAGCCCCAAGGAAAGGCTGAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240613144910_1asn GGTGGGATGAGTACAGGGCCTTCAGGTCTCAG(+AGGACA)GATCCCAAAGCCTCTGTTGCCCCATGGGGAT NGG 10 8 17 8 24 0 100 5 10
    240613144908_nqtg TAGTCATCCCCATGGGGCAACAGAGGCTTTGG(+AGGACA)GATCCTGAGACCTGAAGGCCCTGTACTCATC NGG 10 8 17 8 24 0 100 5 10
    240613144907_f0z2 TTTTCTAAATATCATCAGTCCACATTTGGTTG(+AGGACA)GATCCAGACATCCAGAGCTGGGGGTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613144905_ulbl CAGCCCTCTGCACCCCCAGCTCTGGATGTCTG(+AGGACA)GATCCAACCAAATGTGGACTGATGATATTTA NGG 10 8 17 8 24 0 100 5 10