Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144956_fccm AGACAGAATGCTGTCCAAAAGGGACAAACATG(+AGGACA)GATCCCTAGGGAAGACAACTCCTGAGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613144954_a0ni CCCTGCAAAGCTCAGGAGTTGTCTTCCCTAGG(+AGGACA)GATCCATGTTTGTCCCTTTTGGACAGCATTC NGG 10 8 17 8 24 0 100 5 10
    240613144952_c7m3 GATCTAAGATGCGCTCCTAGGAGCACAGTGGG(+AGGACA)GATCCCAGGCTGTCTGGAGTATGGATAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613144949_cmak CCCCATGGCTATCCATACTCCAGACAGCCTGG(+AGGACA)GATCCCCACTGTGCTCCTAGGAGCGCATCTT NGG 10 8 17 8 24 0 100 5 10
    240613144947_q8r0 TAGGAGCACTGGCTCTCTTGCAGAGGGCCTGG(+AGGACA)GATCCATTTCCAGCACCCAACGTGGCAGCTC NGG 10 8 17 8 24 0 100 5 10
    240613144946_ltuj GTTGTGAGCTGCCACGTTGGGTGCTGGAAATG(+AGGACA)GATCCCAGGCCCTCTGCAAGAGAGCCAGTGC NGG 10 8 17 8 24 0 100 5 10
    240613144944_9mxs TGCAGCTAGGCAGGAGTACTATGGGCCAGGAG(+AGGACA)GATCCCCTGGATACATGGTCCACGGAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240613144942_1xx6 CATGGTGCCCTCCGTGGACCATGTATCCAGGG(+AGGACA)GATCCTCCTGGCCCATAGTACTCCTGCCTAG NGG 10 8 17 8 24 0 100 5 10
    240613144940_ji33 CTGGCACTCATCCTTCTGAGGCTGGGCCACAG(+AGGACA)GATCCAGGTCCCCTTAGCTGTCCCAGGCCCC NGG 10 8 17 8 24 0 100 5 10
    240613144938_f30n CTCCTGGGGCCTGGGACAGCTAAGGGGACCTG(+AGGACA)GATCCTGTGGCCCAGCCTCAGAAGGATGAGT NGG 10 8 17 8 24 0 100 5 10