Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133644_ash4 GCTACCACAGAGCCCCCAGGAGTGTGGCCAGG(+AGGACA)GTACCCACAGCCCTCAGACCCTCTCAATAGC NGG 10 8 17 8 24 0 100 5 10
    240613133642_w2au TTGGGAAGGAGGGCTGTGGTGTGGACCACCTG(+AGGACA)GATCCCTATAGGAGAGACCCTGACTGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613133640_s6wp CCACTGAGCACAGTCAGGGTCTCTCCTATAGG(+AGGACA)GATCCAGGTGGTCCACACCACAGCCCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240613133637_jr4a AGATTGGCATCAGAGGCCCCGACTCCCCCCGG(+AGGACA)GATCCGGGAGCCTAGCCCATCCCTGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613133634_gljj GCGCATGCTGCCAGGGATGGGCTAGGCTCCCG(+AGGACA)GATCCCGGGGGGAGTCGGGGCCTCTGATGCC NGG 10 8 17 8 24 0 100 5 10
    240613133633_unp5 ACATCTGCTCCTGGGGATATACGGGAGAGGAG(+AGGACA)GTACCATAGCATCAAGTTTGGGGAGGACTGT NGG 10 8 17 8 24 0 100 5 10
    240613133632_ejtu GCACCACAGTCCTCCCCAAACTTGATGCTATG(+AGGACA)GTACCTCCTCTCCCGTATATCCCCAGGAGCA NGG 10 8 17 8 24 0 100 5 10
    240613133630_ogfy GCCTGGCAGTGCTGGGAAAGTCTCTGTAAGCG(+AGGACA)GATCCAGAGTGTTCGAGGCACAGCCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240613133629_etmf CAGTGACCCAGGGCTGTGCCTCGAACACTCTG(+AGGACA)GATCCGCTTACAGAGACTTTCCCAGCACTGC NGG 10 8 17 8 24 0 100 5 10
    240613133627_e8eg TGGTAGAACAAGTGTCTTGTGATCAGACACCG(+AGGACA)GATCCCCACCTCAGGCAAAAACCACAAGGAA NGG 10 8 17 8 24 0 100 5 10