Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133538_tuls TCCTGGCTTGTGAACTGGGCCTGTTTGTAGAG(+AGGACA)GATCCTAGAGACGTCCATGCCAGCACTCACC NGG 10 8 17 8 24 0 100 5 10
    240613133536_z454 GTGCTGTGTTGGGAAGCTTTGGAGGTTCCTAG(+AGGACA)GTACCTGGTATCTGGCTCCCAGCAGCAGCTC NGG 10 8 17 8 24 0 100 5 10
    240613133534_63ah AACAGGAGCTGCTGCTGGGAGCCAGATACCAG(+AGGACA)GTACCTAGGAACCTCCAAAGCTTCCCAACAC NGG 10 8 17 8 24 0 100 5 10
    240613133532_cuug TGTTTAGACACATTTTACTAGGCACCTCCGTG(+AGGACA)GATCCTTTACAGTGTTCTTGTGACAAGCTAG NGG 10 8 17 8 24 0 100 5 10
    240613133531_dooi CCACACTAGCTTGTCACAAGAACACTGTAAAG(+AGGACA)GATCCACGGAGGTGCCTAGTAAAATGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240613133530_sou3 GCCATTTCCTTTCCCAACAGAACTCCTCATAG(+AGGACA)GATCCCCTGTCCTTTCCCTGCCCACCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240613133528_ga80 GAGTACAAAAGGTGGGCAGGGAAAGGACAGGG(+AGGACA)GATCCTATGAGGAGTTCTGTTGGGAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613133527_xoj2 ACACAACTCCCCCACAAAAATCACTGGAGTAG(+AGGACA)GATCCCATTAACTACACTCCTCAAATAATCA NGG 10 8 17 8 24 0 100 5 10
    240613133525_q7h2 CAGACTGATTATTTGAGGAGTGTAGTTAATGG(+AGGACA)GATCCTACTCCAGTGATTTTTGTGGGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240613133523_ejir AGGATATTTTCAGTTTGTAATATAAAATTTAG(+AGGACA)GTACCCAGAACCCTCAAAAGACACAACCTCA NGG 10 8 17 8 24 0 100 5 10