Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133522_r3cs ATCCATGAGGTTGTGTCTTTTGAGGGTTCTGG(+AGGACA)GTACCTAAATTTTATATTACAAACTGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613133520_ubql ACCACATGGTGACTCACAACCATCTGTAATGG(+AGGACA)GATCCCATGCCCTTTTCTACTGTGTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240613133519_coix TGTCTTCAGACACACAGTAGAAAAGGGCATGG(+AGGACA)GATCCCATTACAGATGGTTGTGAGTCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613133517_cjbt TTACAGAGACCAGGCAGCCATGGACAGTGAGG(+AGGACA)GTACCCAATGTAACCACAAGGCCAGGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240613133515_byac CTGGGACCTGCCTGGCCTTGTGGTTACATTGG(+AGGACA)GTACCCTCACTGTCCATGGCTGCCTGGTCTC NGG 10 8 17 8 24 0 100 5 10
    240613133514_x63w AAAGGACACTTTCCCATTCCCTGTACCACCAG(+AGGACA)GTACCAGATATGAGCTGGATATATGGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240613133512_jwkk TGAGGCTTGGCCATATATCCAGCTCATATCTG(+AGGACA)GTACCTGGTGGTACAGGGAATGGGAAAGTGT NGG 10 8 17 8 24 0 100 5 10
    240613133509_sj8y TGTAAAACAAGCTCTGACCAGCAACTCCCAGG(+AGGACA)GTACCCACTTCCGCATCACCCACCTGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613133508_3crg TCATCACAGCCAGGTGGGTGATGCGGAAGTGG(+AGGACA)GTACCCTGGGAGTTGCTGGTCAGAGCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613133507_3fua CACCAAGAGCAGAGGTACTGCTGGGATTATAG(+AGGACA)GATCCTACAATTCTCACTGGACCTGGAAGGG NGG 10 8 17 8 24 0 100 5 10