Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133625_m3k8 CCGAGTTCCTTGTGGTTTTTGCCTGAGGTGGG(+AGGACA)GATCCGGTGTCTGATCACAAGACACTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240613133623_vyqn CTTATAGGTCTGAACGCAGGTCCTGCCACCCG(+AGGACA)GTACCACCAGCCTCAGTACAGTCACTACGGC NGG 10 8 17 8 24 0 100 5 10
    240613133622_vvpc CTCAAGCCGTAGTGACTGTACTGAGGCTGGTG(+AGGACA)GTACCGGGTGGCAGGACCTGCGTTCAGACCT NGG 10 8 17 8 24 0 100 5 10
    240613133620_szwo CCATGCGGCTCAGCCATGCTGGTCTCTCCGAG(+AGGACA)GTACCCAGATAGCTGCACCCCTCTGCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240613133618_dwkb AGCAAGGGGAGCAGAGGGGTGCAGCTATCTGG(+AGGACA)GTACCTCGGAGAGACCAGCATGGCTGAGCCG NGG 10 8 17 8 24 0 100 5 10
    240613133616_gsyk ACTGTCTTCAGACACAGCAGAAGAGGGCATCG(+AGGACA)GATCCCATTACAGACTTGAGCCACCCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240613133615_mxj4 AGTACCCACGGGGTGGCTCAAGTCTGTAATGG(+AGGACA)GATCCGATGCCCTCTTCTGCTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613133614_h07c GGCACATGGCCAACCTGTTGGGTTGGTTTTTG(+AGGACA)GTACCTAAGGGACTTTGGAAGAAGGATCAAG NGG 10 8 17 8 24 0 100 5 10
    240613133612_qax2 GGGTTCTTGATCCTTCTTCCAAAGTCCCTTAG(+AGGACA)GTACCAAAAACCAACCCAACAGGTTGGCCAT NGG 10 8 17 8 24 0 100 5 10
    240613133610_xiju AGAGGATACGTGGCTTGAAGCAGCTGCAGAGG(+AGGACA)GTACCTTCAGCCTTGAGCCACACTCTTAAAT NGG 10 8 17 8 24 0 100 5 10