Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133554_nov2 GCCGGGCTCGGCAGTAGGCAGTGAGCTAAGTG(+AGGACA)GATCCCTGTTCTGTGGCTGACCTGAGAGCCC NGG 10 8 17 8 24 0 100 5 10
    240613133553_ucr0 GCCCTCATGATGACAGGGTGCATTTGTAACAG(+AGGACA)GTACCTTGCACACAGTCAGCCCATCTCACAG NGG 10 8 17 8 24 0 100 5 10
    240613133551_envq TCCTACTGTGAGATGGGCTGACTGTGTGCAAG(+AGGACA)GTACCTGTTACAAATGCACCCTGTCATCATG NGG 10 8 17 8 24 0 100 5 10
    240613133550_egdw ATCAGGTTAGCACTGGCTGTAGGCTGGTCTGG(+AGGACA)GATCCTAACAGAATGGCAAGAGGTGAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613133548_ufgk ACTGCTTCCCTCACCTCTTGCCATTCTGTTAG(+AGGACA)GATCCCAGACCAGCCTACAGCCAGTGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240613133547_20b8 AAAGAATTTAAAGGGGGGGGGGGGGTTTCCAG(+AGGACA)GTACCCCTGTCCAGGCTTAAACAAAGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240613133544_5yxr AAGGCTCTTGCTTTGTTTAAGCCTGGACAGGG(+AGGACA)GTACCTGGAAACCCCCCCCCCCCCTTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240613133542_p64j TTCACATCTGAGACAAATAGAAAGCAGCCCAG(+AGGACA)GTACCAATTTGCTCTTCAGTGTCAGTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613133541_kwll TTTTCCTCTGACTGACACTGAAGAGCAAATTG(+AGGACA)GTACCTGGGCTGCTTTCTATTTGTCTCAGAT NGG 10 8 17 8 24 0 100 5 10
    240613133539_x41n GGGTGGGTGAGTGCTGGCATGGACGTCTCTAG(+AGGACA)GATCCTCTACAAACAGGCCCAGTTCACAAGC NGG 10 8 17 8 24 0 100 5 10