Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133609_hcnt TTCTGATTTAAGAGTGTGGCTCAAGGCTGAAG(+AGGACA)GTACCCTCTGCAGCTGCTTCAAGCCACGTAT NGG 10 8 17 8 24 0 100 5 10
    240613133607_s45b CCCCAGTAAAGAAAAGAGAGCAAAAAACACTG(+AGGACA)GTACCCGCCTTGCTTTCTCCTTTATACAGGA NGG 10 8 17 8 24 0 100 5 10
    240613133606_1s97 TGGGATCCTGTATAAAGGAGAAAGCAAGGCGG(+AGGACA)GTACCAGTGTTTTTTGCTCTCTTTTCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240613133604_1dd9 CTGGTACCCGCCTTGCTTTCTCCTTTATACAG(+AGGACA)GATCCCAGCCAAGGGAGTGGTACTAACCATG NGG 10 8 17 8 24 0 100 5 10
    240613133603_chi1 GACCACATGGTTAGTACCACTCCCTTGGCTGG(+AGGACA)GATCCTGTATAAAGGAGAAAGCAAGGCGGGT NGG 10 8 17 8 24 0 100 5 10
    240613133601_vf12 ATGCAGCTCAGAGAGCAGAGGCCCATGCTTTG(+AGGACA)GATCCTTAGCCGAGCTGAGCAAGTCCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613133600_52jk TTTTAAAAGAGGACTTGCTCAGCTCGGCTAAG(+AGGACA)GATCCAAAGCATGGGCCTCTGCTCTCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240613133558_i7ay CTATAGAGGGAAACTAGGGTTCAGAAGAGGGG(+AGGACA)GATCCAAAATCATACAGAGTCCCAGAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613133557_uzp6 TCACTGCTGCTCTGGGACTCTGTATGATTTTG(+AGGACA)GATCCCCCTCTTCTGAACCCTAGTTTCCCTC NGG 10 8 17 8 24 0 100 5 10
    240613133555_af3d AGTGAGGGCTCTCAGGTCAGCCACAGAACAGG(+AGGACA)GATCCACTTAGCTCACTGCCTACTGCCGAGC NGG 10 8 17 8 24 0 100 5 10