Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034048_0nb5 AAACAAGTGCTCTGCCACTGGCTGCATCCCAG(+ATATAT)GATCCTAATAGTAGTCTTTTATAATTCAGTG NGG 10 8 17 8 24 0 100 5 10
    240607034046_l6mj CATTTTCACAAACACGCAAGTTTACATGTAAG(+ATATAT)GATCCAAGGGAAGTCAACGTCCACAGTCCTC NGG 10 8 17 8 24 0 100 5 10
    240607034044_aq2z TGCTCGAGGACTGTGGACGTTGACTTCCCTTG(+ATATAT)GATCCTTACATGTAAACTTGCGTGTTTGTGA NGG 10 8 17 8 24 0 100 5 10
    240607034042_n9nq CCCAGTTAAGAGCACAGGCTGCGCTTCCAAAG(+ATATAT)GATCCAGGTTTGATTCCCAGCACTTACGTGG NGG 10 8 17 8 24 0 100 5 10
    240607034041_uuh9 AGCCCCCACGTAAGTGCTGGGAATCAAACCTG(+ATATAT)GATCCTTTGGAAGCGCAGCCTGTGCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240607034040_8vh2 TAATGGTTTCTTGAGGTCAAGTACTTTTGATG(+ATATAT)GATCCAAACCCTTTCCTAACCCTTCCTCTTC NGG 10 8 17 8 24 0 100 5 10
    240607034039_rogm CGACAGAAGAGGAAGGGTTAGGAAAGGGTTTG(+ATATAT)GATCCATCAAAAGTACTTGACCTCAAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240607034038_sqpj AGCCAACCTCAGGAGGCCTGGTCAGATGACAG(+ATATAT)GATCCAGCTACCCTAGTGTTGGTGCCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240607034036_u87j AGTATTGGGAGGCACCAACACTAGGGTAGCTG(+ATATAT)GATCCTGTCATCTGACCAGGCCTCCTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240607034035_vo47 ATGAAATGGGCAAATTTCTAGACAGATACCAG(+ATATAT)GTACCAAGATTCAATCAGGATCAGATTAATG NGG 10 8 17 8 24 0 100 5 10