Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033949_bdwi AAATCTAGAGAAAACAAGGACAAAACTTTTAG(+ATATAT)GTACCCAAACTTGCTGAAGGCTAATGTCCTA NGG 10 8 17 8 24 0 100 5 10
    240607033948_9xaw GATGTGGAGGCAAGACATGGGTAAAGATTCAG(+ATATAT)GATCCCTCCCTCCCTCAGTAAATGGTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240607033946_e458 CAAGGTTGAAACCATTTACTGAGGGAGGGAGG(+ATATAT)GATCCTGAATCTTTACCCATGTCTTGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240607033944_hwap TTGGTGACTCTGAAGACCAGAAAAGCGTGTTG(+ATATAT)GATCCCAAGAACCAGAGTTGTAGGTGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240607033943_30z9 GGCTCACAACCACCTACAACTCTGGTTCTTGG(+ATATAT)GATCCAACACGCTTTTCTGGTCTTCAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240607033941_hom3 GGGTGGCTTTAGATTTGCTTATTAGCTGAAGG(+ATATAT)GATCCCTCCTGCTTCTGGGATTACAGTCCTC NGG 10 8 17 8 24 0 100 5 10
    240607033940_rbmb CAGCAGAGGACTGTAATCCCAGAAGCAGGAGG(+ATATAT)GATCCCTTCAGCTAATAAGCAAATCTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240607033938_iufn AACCCAGAGGCCAGCCCGCCTGAGTGTGTTGG(+ATATAT)GATCCAATCCCAGCAAGAGCCACCGTTTCCG NGG 10 8 17 8 24 0 100 5 10
    240607033937_9q3x ACACTCGGAAACGGTGGCTCTTGCTGGGATTG(+ATATAT)GATCCCAACACACTCAGGCGGGCTGGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240607033935_902z ACTTGTAACCAGATACAGTTTTACAACTTAGG(+ATATAT)GATCCATGATGAATGAACATTTGGAGAAAAA NGG 10 8 17 8 24 0 100 5 10