Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034019_pclg CCATGTACTCTCCTGCAGACTAGTCTCTGAGG(+ATATAT)GATCCAGGATACAAGTTTGTGTGCTCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240607034018_1bru ATGCAGCAGAAGCAACATAGCTTCTGGGACAG(+ATATAT)GATCCCTTTCAGGCCTACATCTTCCATAGAA NGG 10 8 17 8 24 0 100 5 10
    240607034017_ai1y CAATATTCTATGGAAGATGTAGGCCTGAAAGG(+ATATAT)GATCCTGTCCCAGAAGCTATGTTGCTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240607034014_q5fi TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCCGCAATACCTCTTCTGGGCATATACC NGG 10 8 17 8 24 0 100 5 10
    240607034013_4d0g TTCTGGGTATATGCCCAGAAGAGGTATTGCGG(+ATATAT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607034010_1atl GAAAGGACTATCCAGAGACTGCCCCACCAGGG(+ATATAT)GATCCATCCCATATATAACCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240607034008_rhdd AGTGTCTGGGTTTGGTGGTTATATATGGGATG(+ATATAT)GATCCCCTGGTGGGGCAGTCTCTGGATAGTC NGG 10 8 17 8 24 0 100 5 10
    240607034007_c3kz TGGGGAGCACTAGGGCTCAAAGGAGTGCTCAG(+ATATAT)GATCCAACCAGCTCCCCAAATTTCACGCTGA NGG 10 8 17 8 24 0 100 5 10
    240607034006_jhco TTCCTTCAGCGTGAAATTTGGGGAGCTGGTTG(+ATATAT)GATCCTGAGCACTCCTTTGAGCCCTAGTGCT NGG 10 8 17 8 24 0 100 5 10
    240607034005_pubr TGGTGGGAGGGTGGGAACTGGGGTATTCTTAG(+ATATAT)GTACCTCTGACATTGTAGCAGCTCCTTCAAA NGG 10 8 17 8 24 0 100 5 10