Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034003_cu5m CACCCTTTGAAGGAGCTGCTACAATGTCAGAG(+ATATAT)GTACCTAAGAATACCCCAGTTCCCACCCTCC NGG 10 8 17 8 24 0 100 5 10
    240607034002_nktv CAAAAGACTTTCAGGGGGACCTTCCTTGTTGG(+ATATAT)GATCCAATGGCTGTGGCTGATGGAACCAATC NGG 10 8 17 8 24 0 100 5 10
    240607034000_a0cl TTAAAGATTGGTTCCATCAGCCACAGCCATTG(+ATATAT)GATCCCAACAAGGAAGGTCCCCCTGAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240607033959_o5q3 TGCTCAGGACTCTGCTGTGGGAGGTGTAGCAG(+ATATAT)GATCCTGTCTGTACAGAACATGATAGAATAT NGG 10 8 17 8 24 0 100 5 10
    240607033957_gxwc GCTTCATATTCTATCATGTTCTGTACAGACAG(+ATATAT)GATCCTGCTACACCTCCCACAGCAGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240607033955_bsdj TGACTTAGGGAGACAGACCACCACAGACAGGG(+ATATAT)GATCCTGAGAATCGGGGAGACCCAGGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240607033954_z1kg TGCCATCAAGCCTGGGTCTCCCCGATTCTCAG(+ATATAT)GATCCCCTGTCTGTGGTGGTCTGTCTCCCTA NGG 10 8 17 8 24 0 100 5 10
    240607033953_re1r ACGTGGGAAAGCGTCACAGGTGGCATCCGAAG(+ATATAT)GATCCTTCTGCCATGTGTTGTGCATCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240607033951_i0i1 CCCTGGCTGAGATGCACAACACATGGCAGAAG(+ATATAT)GATCCTTCGGATGCCACCTGTGACGCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240607033950_kbl8 CAAAGTAGGACATTAGCCTTCAGCAAGTTTGG(+ATATAT)GTACCTAAAAGTTTTGTCCTTGTTTTCTCTA NGG 10 8 17 8 24 0 100 5 10