Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033934_3x64 ACTTTTTTTTCTCCAAATGTTCATTCATCATG(+ATATAT)GATCCCTAAGTTGTAAAACTGTATCTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240607033932_qmz2 CCTGGAGCTCTCTGCTCTCTGCATCACCAAGG(+ATATAT)GTACCAGCATAGTCAGGTTTAATCATAATGG NGG 10 8 17 8 24 0 100 5 10
    240607033930_0gl3 CTGAACCATTATGATTAAACCTGACTATGCTG(+ATATAT)GTACCCTTGGTGATGCAGAGAGCAGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240607033929_mn9m TCAAATTTCGGTGTAGATCCAAGCTGGCCTTG(+ATATAT)GATCCATGGATGATGGAGAAAAGAATCAGCA NGG 10 8 17 8 24 0 100 5 10
    240607033927_1c1t AACACTGCTGATTCTTTTCTCCATCATCCATG(+ATATAT)GATCCAAGGCCAGCTTGGATCTACACCGAAA NGG 10 8 17 8 24 0 100 5 10
    240607033925_sv9s TTGTTGGCCAGGCCAGCCTCGAACTCCTAGGG(+ATATAT)GATCCTCAGTCTTCGGCTCTCAGACATGCAG NGG 10 8 17 8 24 0 100 5 10
    240607033924_pojz GGGCCCTGCATGTCTGAGAGCCGAAGACTGAG(+ATATAT)GATCCCCTAGGAGTTCGAGGCTGGCCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240607033922_d4kd GCATATGAGAGCAAGCTGTCAGCAAGGGGCTG(+ATATAT)GTACCTGTCCCTACAAGTTGGCAATAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607033921_wnv5 CATCACCAGCTATTGCCAACTTGTAGGGACAG(+ATATAT)GTACCAGCCCCTTGCTGACAGCTTGCTCTCA NGG 10 8 17 8 24 0 100 5 10
    240607033919_0qb3 GAAGGCAGGGAGGAGTGGCTTAGACAACCGAG(+ATATAT)GATCCAGTTCCCAGGGTCCAGACGGCTGGCT NGG 10 8 17 8 24 0 100 5 10