Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034034_hvv7 TAGATCATTAATCTGATCCTGATTGAATCTTG(+ATATAT)GTACCTGGTATCTGTCTAGAAATTTGCCCAT NGG 10 8 17 8 24 0 100 5 10
    240607034032_mquf GCTCCAGTTTGGGAGGTTCCCAGCAAGGCCTG(+ATATAT)GTACCCTTTCTGTATGCCATCCAAAGTCCTA NGG 10 8 17 8 24 0 100 5 10
    240607034030_13lt ATGCTTAGGACTTTGGATGGCATACAGAAAGG(+ATATAT)GTACCAGGCCTTGCTGGGAACCTCCCAAACT NGG 10 8 17 8 24 0 100 5 10
    240607034029_tj2q GTGTCAGCTAGAGTGTATTATGGGATGCTTAG(+ATATAT)GTACCTGGTGAAACATTGTGTCAGGGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240607034028_jq3h CACAGGCACACCCTGACACAATGTTTCACCAG(+ATATAT)GTACCTAAGCATCCCATAATACACTCTAGCT NGG 10 8 17 8 24 0 100 5 10
    240607034026_b71l AAAGAAGAAGCTTTTGATGAACTAGACTAGTG(+ATATAT)GTACCTCTCATGGCATTTACAGGGTAGACAT NGG 10 8 17 8 24 0 100 5 10
    240607034025_3956 TGGCCATGTCTACCCTGTAAATGCCATGAGAG(+ATATAT)GTACCACTAGTCTAGTTCATCAAAAGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240607034024_ifvd ATGAACACAGTAGGAGTGAAGCTTCTCGTTAG(+ATATAT)GTACCAGCTCGACTTCTTTGTGTTTGATGAC NGG 10 8 17 8 24 0 100 5 10
    240607034023_tnmu CTCATGTCATCAAACACAAAGAAGTCGAGCTG(+ATATAT)GTACCTAACGAGAAGCTTCACTCCTACTGTG NGG 10 8 17 8 24 0 100 5 10
    240607034021_v30i GGGATCAGGGGAGCACACAAACTTGTATCCTG(+ATATAT)GATCCCTCAGAGACTAGTCTGCAGGAGAGTA NGG 10 8 17 8 24 0 100 5 10