Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003852_ghbq TGCACACAACATCCATATCTCAGGACACCTTG(+ATATAT)GTACCACTAGAGCAGCCAGGATCTAGGTCTG NGG 10 8 17 8 24 0 100 5 10
    240607003851_eh3f GTGTTATATGTTAAAACTTTCTCTTTAGCAAG(+ATATAT)GTACCAAGAAATTCACCTGTTCCTTCAGATA NGG 10 8 17 8 24 0 100 5 10
    240607003850_4vz1 GCCCATATCTGAAGGAACAGGTGAATTTCTTG(+ATATAT)GTACCTTGCTAAAGAGAAAGTTTTAACATAT NGG 10 8 17 8 24 0 100 5 10
    240607003848_jpr0 AGATTATATAGAAACAATCTGTACTGCTCTGG(+ATATAT)GATCCTTTGGTCTTAGAAACCATTTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240607003846_6zdr CACCCCCCCAAAAATGGTTTCTAAGACCAAAG(+ATATAT)GATCCCAGAGCAGTACAGATTGTTTCTATAT NGG 10 8 17 8 24 0 100 5 10
    240607003845_6t8i TTCATGATTTCTTATCATGTTACCATAGCAAG(+ATATAT)GATCCAGGTCCTTGCTGGAGTCAGTGGTCAT NGG 10 8 17 8 24 0 100 5 10
    240607003844_2jwk GTCACATGACCACTGACTCCAGCAAGGACCTG(+ATATAT)GATCCTTGCTATGGTAACATGATAAGAAATC NGG 10 8 17 8 24 0 100 5 10
    240607003842_lxgv ATTAGTGTAAAGGTATCTCACCCTTAGATCTG(+ATATAT)GATCCCTTGGAACTGGAGTTACAATTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240607003840_zeq6 GGCAGCTTACAATTGTAACTCCAGTTCCAAGG(+ATATAT)GATCCAGATCTAAGGGTGAGATACCTTTACA NGG 10 8 17 8 24 0 100 5 10
    240607003839_ib85 GTAACAGACCATGTGAAAGCTCACACTGCTTG(+ATATAT)GATCCACTGCCTGGGCGGGCGGCTCCAGGCT NGG 10 8 17 8 24 0 100 5 10