Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003748_knyl CTTCTATTCAGCCCCAAGAGGGTCCTCTCGTG(+ATATAT)GTACCTCATGTTTTACATTGAAACCAGCACC NGG 10 8 17 8 24 0 100 5 10
    240607003746_4ew3 GGTACTGTTTTGTACTATATAATGACTTAGAG(+ATATAT)GTACCAGGATGATGTCCTGGAAACACGGGTC NGG 10 8 17 8 24 0 100 5 10
    240607003745_0lzy TATCAGACCCGTGTTTCCAGGACATCATCCTG(+ATATAT)GTACCTCTAAGTCATTATATAGTACAAAACA NGG 10 8 17 8 24 0 100 5 10
    240607003744_6qi6 ACTAGATATCCACATATTAGAGAATGAAACTG(+ATATAT)GATCCCTAAAATGTACCACATGCAAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240607003742_vbk8 TTGATTCATTTTTGCATGTGGTACATTTTAGG(+ATATAT)GATCCAGTTTCATTCTCTAATATGTGGATAT NGG 10 8 17 8 24 0 100 5 10
    240607003741_j6z0 TTAGAACTTTTAAAAAATAAACTTTCTTTTTG(+ATATAT)GTACCTCTGGCTTCCTATATATGTCTTGGAA NGG 10 8 17 8 24 0 100 5 10
    240607003739_q5z7 AATGTTTCCAAGACATATATAGGAAGCCAGAG(+ATATAT)GTACCAAAAAGAAAGTTTATTTTTTAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240607003738_05xd GGTAACTTCATTACAGTGTCTATAAAGTTGTG(+ATATAT)GATCCCAGTCTTGGTGCCAAATGCATATAAA NGG 10 8 17 8 24 0 100 5 10
    240607003736_7b04 AGCTTTTTATATGCATTTGGCACCAAGACTGG(+ATATAT)GATCCACAACTTTATAGACACTGTAATGAAG NGG 10 8 17 8 24 0 100 5 10
    240607003735_90np AGGATTCATAGTTGATCATCTTCCAGTTGATG(+ATATAT)GTACCAACTAGATCTTATAAGTACTAGATAT NGG 10 8 17 8 24 0 100 5 10