Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003719_15vn ATGCTCCTGACTCCAGATGAGTTCTAGGCTGG(+ATATAT)GATCCTTTAATCCTTCAAAGAAGCAGTTAGT NGG 10 8 17 8 24 0 100 5 10
    240607003716_9qqn CCTCCTGGGAAGAAAACTTTTGGCTCCCCCTG(+ATATAT)GTACCTCAGCAGCGGCATTGCATAGGATTTC NGG 10 8 17 8 24 0 100 5 10
    240607003715_d1ij CTTTTGAAATCCTATGCAATGCCGCTGCTGAG(+ATATAT)GTACCAGGGGGAGCCAAAAGTTTTCTTCCCA NGG 10 8 17 8 24 0 100 5 10
    240607003714_wr84 TCCATTTAAACCCTTTATATTTCAACAGTGAG(+ATATAT)GATCCTTTCCCATGAAGTATTTTCAGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240607003713_knp1 CCGTGAGACACTGAAAATACTTCATGGGAAAG(+ATATAT)GATCCTCACTGTTGAAATATAAAGGGTTTAA NGG 10 8 17 8 24 0 100 5 10
    240607003711_8zzx TTGGGATGGGAGAGACAATAAATGTTTTAGTG(+ATATAT)GTACCATCTCTATATTCGTCTTCTTTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240607003710_qd7l TCCTCTCTGCAAAGAAGACGAATATAGAGATG(+ATATAT)GTACCACTAAAACATTTATTGTCTCTCCCAT NGG 10 8 17 8 24 0 100 5 10
    240607003708_6ayl CAAAAGGAGAAAAAGATTGATGTGTGAGTAGG(+ATATAT)GTACCACCCAGATCGCAGTCTGACTTCCTTA NGG 10 8 17 8 24 0 100 5 10
    240607003706_r1x8 CTTCCTAAGGAAGTCAGACTGCGATCTGGGTG(+ATATAT)GTACCCTACTCACACATCAATCTTTTTCTCC NGG 10 8 17 8 24 0 100 5 10
    240607003705_dj2q GATGGATGTAGTCATGTCCATTTAGAGGCAGG(+ATATAT)GTACCACATGTCACAGGCAGCCCTGAGTGTG NGG 10 8 17 8 24 0 100 5 10