Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003733_3dhz TGTGGATATCTAGTACTTATAAGATCTAGTTG(+ATATAT)GTACCATCAACTGGAAGATGATCAACTATGA NGG 10 8 17 8 24 0 100 5 10
    240607003731_h51p TGGGTGCCCATAGAGGTTACAAGGGGCCATTG(+ATATAT)GATCCTTTTGACCTGGAGTTGAGGAATGTTA NGG 10 8 17 8 24 0 100 5 10
    240607003730_y2ov AAAACTAACATTCCTCAACTCCAGGTCAAAAG(+ATATAT)GATCCAATGGCCCCTTGTAACCTCTATGGGC NGG 10 8 17 8 24 0 100 5 10
    240607003729_qfcg TCTAGAAACATGTCGATGGAAGAGAAACTTTG(+ATATAT)GATCCCATGCCTCCTGTTTACAGTAGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240607003727_n29x AGAAGAAAAGCTACTGTAAACAGGAGGCATGG(+ATATAT)GATCCAAAGTTTCTCTTCCATCGACATGTTT NGG 10 8 17 8 24 0 100 5 10
    240607003726_4jbm AGTAACTTTGCAGTTTTTGTAGTTAAAATATG(+ATATAT)GATCCAAAAGGTATATATGCATGGCGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240607003725_l0fk GACCAGGACACGCCATGCATATATACCTTTTG(+ATATAT)GATCCATATTTTAACTACAAAAACTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240607003724_4pwz CATCCTAAACACAGCTTCTCTGTGTGTAAAAG(+ATATAT)GTACCTGCTATTCCTAAGGGGCTGCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240607003722_vvg2 ATGTTGCCTGTGCAGCCCCTTAGGAATAGCAG(+ATATAT)GTACCTTTTACACACAGAGAAGCTGTGTTTA NGG 10 8 17 8 24 0 100 5 10
    240607003721_b1wa TGAAGACTAACTGCTTCTTTGAAGGATTAAAG(+ATATAT)GATCCCAGCCTAGAACTCATCTGGAGTCAGG NGG 10 8 17 8 24 0 100 5 10