Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003821_bbkj AGGAAAGGAAAACATGTTTTAACATGACTGAG(+ATATAT)GTACCCAGGTCACTTGGTGGTCCCTTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240607003819_dhyx TTCATTTATGATTGGCTAACAAGTAACACCTG(+ATATAT)GATCCCACAAGGGACCACCAAGTGACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607003817_ojhj AGGTACCCAGGTCACTTGGTGGTCCCTTGTGG(+ATATAT)GATCCAGGTGTTACTTGTTAGCCAATCATAA NGG 10 8 17 8 24 0 100 5 10
    240607003815_9n5j TGTCTTTTGGCTAATCCTAAGTAGCTCCTTTG(+ATATAT)GATCCTCCTTCACAGGCTCTGTAGCTGTAGA NGG 10 8 17 8 24 0 100 5 10
    240607003814_f3e7 AGCCATCTACAGCTACAGAGCCTGTGAAGGAG(+ATATAT)GATCCAAAGGAGCTACTTAGGATTAGCCAAA NGG 10 8 17 8 24 0 100 5 10
    240607003812_rq85 TCGCAAAACTTGGTCTCTGCTGGAGAACACTG(+ATATAT)GATCCCCTGTGGGTGGGTGGGTGGGCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240607003810_yzpj ATTTTCAACAGCCCACCCACCCACCCACAGGG(+ATATAT)GATCCAGTGTTCTCCAGCAGAGACCAAGTTT NGG 10 8 17 8 24 0 100 5 10
    240607003809_zc0c AGAGAGCCCTTTACATCCACTCTGTTGGGCAG(+ATATAT)GATCCAGTCAACCTGCTCAGCCTGCTTAGGC NGG 10 8 17 8 24 0 100 5 10
    240607003807_hh2d GCTGAGCCTAAGCAGGCTGAGCAGGTTGACTG(+ATATAT)GATCCTGCCCAACAGAGTGGATGTAAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240607003804_attp TGTTCCCCCATTGCACACTGGAGAAGCCCTGG(+ATATAT)GTACCGAGTGTGGGGACCCCATGGACAGTAC NGG 10 8 17 8 24 0 100 5 10