Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003914_g5tc ACCTCGAGGCCTGACAGCCCTGTGCTGCCTGG(+ATATAT)GATCCTAAGGGTTCCTATGTGGATTAACCTA NGG 10 8 17 8 24 0 100 5 10
    240607003913_vjja ATCGATAGGTTAATCCACATAGGAACCCTTAG(+ATATAT)GATCCCAGGCAGCACAGGGCTGTCAGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240607003911_yf1k TCCCACTTAACCTTCATTCTTTGACAAATGAG(+ATATAT)GTACCTCCTGTAAATGGGGAGTCTTTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240607003910_b5z9 TCATGAGAGGAAAGACTCCCCATTTACAGGAG(+ATATAT)GTACCTCATTTGTCAAAGAATGAAGGTTAAG NGG 10 8 17 8 24 0 100 5 10
    240607003907_cbh1 GTCCAGAGCCTGTTTCCTCAGGGCTCCCCTGG(+ATATAT)GTACCCAGCTCCCAGGGTAGGTGCCCACCTG NGG 10 8 17 8 24 0 100 5 10
    240607003905_owgn GAGTTCAGGTGGGCACCTACCCTGGGAGCTGG(+ATATAT)GTACCCAGGGGAGCCCTGAGGAAACAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240607003904_m83r CTTGGATGTTGGGAGTGTAGCAGGCACAGGAG(+ATATAT)GTACCTCTGCAGAAGGAAGGCGGAGGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240607003903_uclw AGGCCTCCACCCTCCGCCTTCCTTCTGCAGAG(+ATATAT)GTACCTCCTGTGCCTGCTACACTCCCAACAT NGG 10 8 17 8 24 0 100 5 10
    240607003902_h59y TGTCCGTTTCCCCTCACTGTCAGGGAAAGCTG(+ATATAT)GATCCCTCTGGCTGACTTAGAGTACTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240607003900_2ip5 CCCAAGTCACAGTACTCTAAGTCAGCCAGAGG(+ATATAT)GATCCAGCTTTCCCTGACAGTGAGGGGAAAC NGG 10 8 17 8 24 0 100 5 10