Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003803_9c37 TTATGGTACTGTCCATGGGGTCCCCACACTCG(+ATATAT)GTACCCAGGGCTTCTCCAGTGTGCAATGGGG NGG 10 8 17 8 24 0 100 5 10
    240607003801_1dcl CCCATCCAAAGCCATCGGTGGAACTTCTGGGG(+ATATAT)GATCCTTTGCCAAAACCCCAAGGCAAAATCC NGG 10 8 17 8 24 0 100 5 10
    240607003759_1bia ACCTTGGATTTTGCCTTGGGGTTTTGGCAAAG(+ATATAT)GATCCCCCAGAAGTTCCACCGATGGCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240607003758_vcma CACTGGGAGTGTTTGGATTAGGGTCCCAGCTG(+ATATAT)GTACCCCACCTTATTTAGTCCAATGAGACCA NGG 10 8 17 8 24 0 100 5 10
    240607003756_j61o CCGTTTGGTCTCATTGGACTAAATAAGGTGGG(+ATATAT)GTACCAGCTGGGACCCTAATCCAAACACTCC NGG 10 8 17 8 24 0 100 5 10
    240607003755_4fl1 CCACTGACCTGCATTTTGGTGACTGGAGAATG(+ATATAT)GATCCTTCATCTGGGCTAGGAGATCCGTGCC NGG 10 8 17 8 24 0 100 5 10
    240607003754_6nxd ACAGTGGCACGGATCTCCTAGCCCAGATGAAG(+ATATAT)GATCCATTCTCCAGTCACCAAAATGCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240607003752_wp5q AGGCTGAAACCAATCCAGAGCACTTCCCACTG(+ATATAT)GATCCATTTCAACAGCTGCCTTGCAAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240607003751_k40e TGACTTCCTTTTGCAAGGCAGCTGTTGAAATG(+ATATAT)GATCCAGTGGGAAGTGCTCTGGATTGGTTTC NGG 10 8 17 8 24 0 100 5 10
    240607003750_xfk3 TTTGCGGTGCTGGTTTCAATGTAAAACATGAG(+ATATAT)GTACCACGAGAGGACCCTCTTGGGGCTGAAT NGG 10 8 17 8 24 0 100 5 10