Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003703_6mbd TGGCACACACTCAGGGCTGCCTGTGACATGTG(+ATATAT)GTACCCTGCCTCTAAATGGACATGACTACAT NGG 10 8 17 8 24 0 100 5 10
    240607003701_h6gj TAGGCAGCTCACAACTGTATTCTAGCTTTGGG(+ATATAT)GATCCAATATTGTCTTCTGGTCTCTGTGGCC NGG 10 8 17 8 24 0 100 5 10
    240607003700_p2ep GTAGTGGCCACAGAGACCAGAAGACAATATTG(+ATATAT)GATCCCCAAAGCTAGAATACAGTTGTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240607003659_n783 TAAGGAACAACTAGAATTTAGTAACATTTGAG(+ATATAT)GATCCCAGTGTGGGTCTGAGGAAGGATTCTT NGG 10 8 17 8 24 0 100 5 10
    240607003657_98ex GCCCTAAGAATCCTTCCTCAGACCCACACTGG(+ATATAT)GATCCTCAAATGTTACTAAATTCTAGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240607003656_995t AACTACTGGGTTGAGATATTTTCTAAAATCTG(+ATATAT)GATCCCTAAACATCCCAATGTGCTGAATAAA NGG 10 8 17 8 24 0 100 5 10
    240607003654_xv8v AAGTATTTATTCAGCACATTGGGATGTTTAGG(+ATATAT)GATCCAGATTTTAGAAAATATCTCAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240607003653_n2kd GCTTAGAACCATCTGTAACTCTAGTTCCAGAG(+ATATAT)GATCCAGTGTTTTCTGGCCTCCATAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240607003652_dp7j TGCCTGCTCTCTATGGAGGCCAGAAAACACTG(+ATATAT)GATCCTCTGGAACTAGAGTTACAGATGGTTC NGG 10 8 17 8 24 0 100 5 10
    240607003650_juif CTTCCCTCCTGACCTCAAGCCATGTAGAGTTG(+ATATAT)GATCCCAAAAGCCCTGAGCAATGGCACTTTC NGG 10 8 17 8 24 0 100 5 10