Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003838_tgj8 ACAGCAGCCTGGAGCCGCCCGCCCAGGCAGTG(+ATATAT)GATCCAAGCAGTGTGAGCTTTCACATGGTCT NGG 10 8 17 8 24 0 100 5 10
    240607003836_9zse TGTAAGAATGTGGCCAAGGTGGTACAGCCTTG(+ATATAT)GTACCATAATGCAAGACACAGTGACAATGTC NGG 10 8 17 8 24 0 100 5 10
    240607003834_oa4h AACTCGACATTGTCACTGTGTCTTGCATTATG(+ATATAT)GTACCAAGGCTGTACCACCTTGGCCACATTC NGG 10 8 17 8 24 0 100 5 10
    240607003833_5zdr CCAGGCCACAAACCTGCCTGTGGGGCACTGAG(+ATATAT)GATCCTAGAGGATGCCATCTTCAGGTAGGGG NGG 10 8 17 8 24 0 100 5 10
    240607003832_tvnm CTCCTCCCCTACCTGAAGATGGCATCCTCTAG(+ATATAT)GATCCTCAGTGCCCCACAGGCAGGTTTGTGG NGG 10 8 17 8 24 0 100 5 10
    240607003831_e0py GGATGGAACTTGAGAATATCATCCTGAGTGAG(+ATATAT)GTACCCCGGAGCCAGCAAGACAGATGATATA NGG 10 8 17 8 24 0 100 5 10
    240607003828_028h GAGTATATATCATCTGTCTTGCTGGCTCCGGG(+ATATAT)GTACCTCACTCAGGATGATATTCTCAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240607003826_jb5s AACTAAGCCACTGAAATCGAATTGAATCAGGG(+ATATAT)GATCCCAAATAAAAAGATCATATTAAGGCAA NGG 10 8 17 8 24 0 100 5 10
    240607003825_iw1g AGAATTTGCCTTAATATGATCTTTTTATTTGG(+ATATAT)GATCCCCTGATTCAATTCGATTTCAGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240607003822_nqsz TGGATCCCACAAGGGACCACCAAGTGACCTGG(+ATATAT)GTACCTCAGTCATGTTAAAACATGTTTTCCT NGG 10 8 17 8 24 0 100 5 10