Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606231020_703e GAGCTTAAAAGACACTCGTTAGAGTGCACCTG(+ATATAT)GATCCTGCCATGACATCACAGGCGAAGCATC NGG 10 8 17 8 24 0 100 5 10
    240606231018_esjj CGGCTGATGCTTCGCCTGTGATGTCATGGCAG(+ATATAT)GATCCAGGTGCACTCTAACGAGTGTCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606231017_bzyh AAATGACCAGGCTCCTGGTAAATGGAAATCAG(+ATATAT)GATCCAAGCAACTTTGAATTCTGGGAAATGA NGG 10 8 17 8 24 0 100 5 10
    240606231015_u1jw CAAAGTCATTTCCCAGAATTCAAAGTTGCTTG(+ATATAT)GATCCTGATTTCCATTTACCAGGAGCCTGGT NGG 10 8 17 8 24 0 100 5 10
    240606231012_sndf CTCACTACCATCTCTTAACTCCAGTCCCAGGG(+ATATAT)GATCCAGTGCCATCTTTTGGCCTCCCCAAGC NGG 10 8 17 8 24 0 100 5 10
    240606231011_i4y8 CTGGTGCTTGGGGAGGCCAAAAGATGGCACTG(+ATATAT)GATCCCCTGGGACTGGAGTTAAGAGATGGTA NGG 10 8 17 8 24 0 100 5 10
    240606231008_ajvp AGGAGGGTAGAATTTGAAAACCTCCTCCCGAG(+ATATAT)GATCCAATGGTTCTTTCTGCAGAATGCCTGC NGG 10 8 17 8 24 0 100 5 10
    240606231006_00r5 GTAGGGCAGGCATTCTGCAGAAAGAACCATTG(+ATATAT)GATCCTCGGGAGGAGGTTTTCAAATTCTACC NGG 10 8 17 8 24 0 100 5 10
    240606231002_6573 GGATGACAGAACCATTTCAGTGCAAACCCCAG(+ATATAT)GATCCAAGGCTGCAAGCCTAAGTCACAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606231000_814x GGTCAGACTTGTGACTTAGGCTTGCAGCCTTG(+ATATAT)GATCCTGGGGTTTGCACTGAAATGGTTCTGT NGG 10 8 17 8 24 0 100 5 10