Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606230919_z7zx TCATTTTGTTTCCTTTTAAGAAATTGCCACAG(+ATATAT)GTACCCCTACGTTTAGTAACTACCATGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606230917_bw00 ACTTATCAGCATGGTAGTTACTAAACGTAGGG(+ATATAT)GTACCTGTGGCAATTTCTTAAAAGGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240606230914_a6rm TATATTTCCCTTTCCCATTCAATCAATAGGTG(+ATATAT)GATCCAAGCTTGTTAGCTACTCAGGACTTGC NGG 10 8 17 8 24 0 100 5 10
    240606230913_nf5r CTACTGCAAGTCCTGAGTAGCTAACAAGCTTG(+ATATAT)GATCCACCTATTGATTGAATGGGAAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240606230910_puww CAAGTTCCAAGCCAACCAGGGGTACAAAATGG(+ATATAT)GATCCTGACTTTAAAATTGAACAATTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606230908_u9d4 GTCATTTTTAAATTGTTCAATTTTAAAGTCAG(+ATATAT)GATCCCATTTTGTACCCCTGGTTGGCTTGGA NGG 10 8 17 8 24 0 100 5 10
    240606230906_13z6 TAAAATCTGAGGTAGGGAAAGGTAAAAAGCAG(+ATATAT)GTACCCTCTCTTCAAATAGAAGCGCATGTTC NGG 10 8 17 8 24 0 100 5 10
    240606230904_f20o ACAAGGAACATGCGCTTCTATTTGAAGAGAGG(+ATATAT)GTACCTGCTTTTTACCTTTCCCTACCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606230902_vk9d AGTACTCACATTGCTACTGTCTTCTGTTGTAG(+ATATAT)GTACCATGCTCGGAGCTTGCTTACAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240606230901_01re AAATTTTTATTTGTAAGCAAGCTCCGAGCATG(+ATATAT)GTACCTACAACAGAAGACAGTAGCAATGTGA NGG 10 8 17 8 24 0 100 5 10