Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606230938_4t03 ATACCTTCCAAGGACCAGTAGACTTGAATACG(+ATATAT)GATCCAATGGGAGTTGGTTGTCTGGTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240606230936_m6l0 TCATCTTGACACCAGACAACCAACTCCCATTG(+ATATAT)GATCCGTATTCAAGTCTACTGGTCCTTGGAA NGG 10 8 17 8 24 0 100 5 10
    240606230934_s4vq GACTCCCTCCAGGGGTTCCCAGCTCCCACAGG(+ATATAT)GTACCTGCCATCTCCATCCCTGGACTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606230933_jn8u GTGGCTCTGCAGTCCAGGGATGGAGATGGCAG(+ATATAT)GTACCCTGTGGGAGCTGGGAACCCCTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606230931_ukh1 ATTTTGGAAGAACACAGAAAGTTTTCAGACAG(+ATATAT)GATCCATTTCCCCAGTACTCAACCAAAATTG NGG 10 8 17 8 24 0 100 5 10
    240606230930_xpib CTTTCCAATTTTGGTTGAGTACTGGGGAAATG(+ATATAT)GATCCTGTCTGAAAACTTTCTGTGTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606230927_lrlp CCAAGAGGATGGGATTCATATTTAGTCCCTAG(+ATATAT)GTACCGTTAAGAAGTGGCACACTATTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240606230925_1jtw ATATACTTACAATAGTGTGCCACTTCTTAACG(+ATATAT)GTACCTAGGGACTAAATATGAATCCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240606230924_7di8 TCATGCAGTGTCACCCGTGTCACGCAGCGCGG(+ATATAT)GTACCCTGAGAGGACTCTGAATACCCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606230921_xqnn AGTTGGGGAAGGGTATTCAGAGTCCTCTCAGG(+ATATAT)GTACCCGCGCTGCGTGACACGGGTGACACTG NGG 10 8 17 8 24 0 100 5 10