Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606230957_fwmi AGTCATGTTCTATTTCAGTTTTAAATGCCCAG(+ATATAT)GTACCCAAGGAATGCACCCTTCTCCTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240606230954_ldgl GTGCGTGGCCAGGAGAAGGGTGCATTCCTTGG(+ATATAT)GTACCTGGGCATTTAAAACTGAAATAGAACA NGG 10 8 17 8 24 0 100 5 10
    240606230953_6gom ACAACACAAGAAAACTTAAAAGAATGTGTATG(+ATATAT)GTACCGTGGATCTTTCTGTGGTGGTTCATTC NGG 10 8 17 8 24 0 100 5 10
    240606230951_fjhj CTGTGGAATGAACCACCACAGAAAGATCCACG(+ATATAT)GTACCATACACATTCTTTTAAGTTTTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240606230949_ncbq ACTGAAAAGAAAAGGAATGAAGAAGACTTCTG(+ATATAT)GATCCAAAGCCTTAAGAGAGTCTACGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240606230948_btjg TGTTAGCACACGTAGACTCTCTTAAGGCTTTG(+ATATAT)GATCCAGAAGTCTTCTTCATTCCTTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606230946_2asm TGCGTTCCTGCAGTGTCCTCCTCGAGGAGCAG(+ATATAT)GATCCACATTTTCAAGAATGTCCTGACTCCT NGG 10 8 17 8 24 0 100 5 10
    240606230945_3re0 TCACGAGGAGTCAGGACATTCTTGAAAATGTG(+ATATAT)GATCCTGCTCCTCGAGGAGGACACTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606230943_2x05 AGCACATGTCAAGAAGGACTCAGGAAAGAAAG(+ATATAT)GTACCTGTGCCTTGCCCCAGTGTCTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240606230942_q6bj TGAACTCAGACAGACACTGGGGCAAGGCACAG(+ATATAT)GTACCTTTCTTTCCTGAGTCCTTCTTGACAT NGG 10 8 17 8 24 0 100 5 10