Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606230859_aei0 GACATTTTTACATTAGCAAAGTCACCTCATGG(+ATATAT)GATCCTGCAGTTAAAGTGTTAAGACGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606230858_3w9r TCTGCAGCCTCGTCTTAACACTTTAACTGCAG(+ATATAT)GATCCCATGAGGTGACTTTGCTAATGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606230856_0raa GTCACTGAGAACTTTGAGCACCCTGGGACGAG(+ATATAT)GTACCCCCACCTCAATGATATTTCTCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240606230853_tg8z GTTGTTCTCAGAGAAATATCATTGAGGTGGGG(+ATATAT)GTACCTCGTCCCAGGGTGCTCAAAGTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606230852_evm6 TTCTAATTCCTGGAAATTGTGTGGGTGGAGTG(+ATATAT)GATCCCTGCACTGGGGGGAATAACATGGACC NGG 10 8 17 8 24 0 100 5 10
    240606230850_uoip TCAGTGGTCCATGTTATTCCCCCCAGTGCAGG(+ATATAT)GATCCACTCCACCCACACAATTTCCAGGAAT NGG 10 8 17 8 24 0 100 5 10
    240606230848_lh9u GAAAACCACTAAGAATCTTCCAGCACTGTATG(+ATATAT)GATCCTCAATGTTTCTATCTTATTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606230846_w15p CATTTCCTTTACAATAAGATAGAAACATTGAG(+ATATAT)GATCCATACAGTGCTGGAAGATTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606230844_14a4 GAAAACCACTAAGAATCTTCCAGCACTGTATG(+ATATAT)GATCCTCAATGTTTCTATCTTATTGTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606230843_4i29 CATTTCCTTTACAATAAGATAGAAACATTGAG(+ATATAT)GATCCATACAGTGCTGGAAGATTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10