Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173453_anwl AGGCTGTTCTGTTCTGTTTAACAGAACAGAAG(+CAGTGT)GATCCCAGGGCCCGGCTCCTGTTCTCCATGT NGG 10 8 17 8 24 0 100 5 10
    240605173451_ps9m TAGACTCTGCCCTCGTCTTGCTCTCCAGGTTG(+CAGTGT)GTACCTCAGCCCCTCTATTGTGAACTTAGAT NGG 10 8 17 8 24 0 100 5 10
    240605173450_s764 TCCCTATCTAAGTTCACAATAGAGGGGCTGAG(+CAGTGT)GTACCAACCTGGAGAGCAAGACGAGGGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605173448_yrzk CAGCCCCCAGCCCGGGAATGTCTGTTGCAGGG(+CAGTGT)GATCCTCTGTCTCCAGCCCTGGCAAGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240605173447_j2u3 TGGCAGGGACCTTGCCAGGGCTGGAGACAGAG(+CAGTGT)GATCCCCTGCAACAGACATTCCCGGGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605173445_s0xf GACCTTCTGCACTCCATCCATAATAGCCTTTG(+CAGTGT)GTACCTTCCAGAGGAGTCCTTCCCGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605173444_5v41 TTGCCAGCTCTCGGGAAGGACTCCTCTGGAAG(+CAGTGT)GTACCAAAGGCTATTATGGATGGAGTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605173442_kf6s AGAAAGCACCAGGTCAGGCAGGGCCTGAGGTG(+CAGTGT)GATCCGAGCTGGGTCCAGGGCTCGAATCTCA NGG 10 8 17 8 24 0 100 5 10
    240605173441_e34t TGGTGTGAGATTCGAGCCCTGGACCCAGCTCG(+CAGTGT)GATCCACCTCAGGCCCTGCCTGACCTGGTGC NGG 10 8 17 8 24 0 100 5 10
    240605173439_cj99 GAGACCCCGAGGGAGGTGAGCTTGAAGTGAGG(+CAGTGT)GTACCATCCAAGGTACTCCAGGACCCTCTCC NGG 10 8 17 8 24 0 100 5 10