Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173350_6s84 GGGACAGCTAAGGAGATGGCCGTGGACTGTTG(+CAGTGT)GATCCCTGAGGTTGGGCTGGGCTCTTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605173349_v71g TGTCCCTCTGACTGGATACAACCTAGTAAATG(+CAGTGT)GTACCTTCCAGCTGGGCCTGTTTCTGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605173347_b6k0 GAAGGAGCAGCAGAAACAGGCCCAGCTGGAAG(+CAGTGT)GTACCATTTACTAGGTTGTATCCAGTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240605173346_rrae TTGTCCCCAGGATACAGCAGATGCTCAACAGG(+CAGTGT)GTACCAGAGACACTGTCTGTCTGGAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605173345_zk7t CCGCCAGAGCCTCCAGACAGACAGTGTCTCTG(+CAGTGT)GTACCCTGTTGAGCATCTGCTGTATCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605173343_nsqd TTTTCCTTTCCAGAAACATGTTTGGGGCCGTG(+CAGTGT)GATCCGAACAGGCAACAGGGGTGCTCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240605173342_z5yc ATCTTTGGCAGAGCACCCCTGTTGCCTGTTCG(+CAGTGT)GATCCACGGCCCCAAACATGTTTCTGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605173341_a0d1 CAAGGAAGGGCCCTCTGTCCCCTGATATGCTG(+CAGTGT)GTACCATTGTTTGTGTCCAGGGGATGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605173339_hds4 GGTGAGCTGGCATCCCCTGGACACAAACAATG(+CAGTGT)GTACCAGCATATCAGGGGACAGAGGGCCCTT NGG 10 8 17 8 24 0 100 5 10
    240605173338_iaw7 CAGGCCAGGACCCACACAACTGATGGGGGCAG(+CAGTGT)GATCCCCAGTGATGGGTCCAGAGTGGGAATC NGG 10 8 17 8 24 0 100 5 10