Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173336_sgli GACTTGATTCCCACTCTGGACCCATCACTGGG(+CAGTGT)GATCCTGCCCCCATCAGTTGTGTGGGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605173335_6huk GGTCTCCCGAGAGCATGCCGAGTGTCCTGAGG(+CAGTGT)GATCCTCGGCTAGCCCCCATCTTCTACTTGA NGG 10 8 17 8 24 0 100 5 10
    240605173333_4rem AGCCTTCAAGTAGAAGATGGGGGCTAGCCGAG(+CAGTGT)GATCCCTCAGGACACTCGGCATGCTCTCGGG NGG 10 8 17 8 24 0 100 5 10
    240605173331_6pz4 GGAGGGAACCTTGGCAGCACTGGGCCTGTAGG(+CAGTGT)GATCCAGAGTTCTCAGGCAATAGAAGTGATC NGG 10 8 17 8 24 0 100 5 10
    240605173330_g18g ACCCAGATCACTTCTATTGCCTGAGAACTCTG(+CAGTGT)GATCCCTACAGGCCCAGTGCTGCCAAGGTTC NGG 10 8 17 8 24 0 100 5 10
    240605173328_bgu7 GAGGGTGGTCTGTCGCATCACCACATTAGTGG(+CAGTGT)GTACCTGACAGTTACCTCATTGGCAGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605173327_eqwz CTCACCTTGGCTGCCAATGAGGTAACTGTCAG(+CAGTGT)GTACCCACTAATGTGGTGATGCGACAGACCA NGG 10 8 17 8 24 0 100 5 10
    240605173325_rjj9 CTGCCCCTCTGACTCCTGCCCAGAAGTGGTGG(+CAGTGT)GTACCCCCCCACCCCGTGCCATTCATTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605173323_7o5h GGGTGGGACAATGAATGGCACGGGGTGGGGGG(+CAGTGT)GTACCCACCACTTCTGGGCAGGAGTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605173321_prfw GAGCTTCTTTGGTATCACCTGTCTATGTGCAG(+CAGTGT)GATCCTTCCAAAATGGAAGTAAACTACGCAG NGG 10 8 17 8 24 0 100 5 10