Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173423_e2vl GCAGGCTGCCCACAGCCGCAGACATGGACAGG(+CAGTGT)GTACCTGTGACTCCCTTGTAACTGTCCCCAA NGG 10 8 17 8 24 0 100 5 10
    240605173421_7pr5 CAAGTCAGAATTCCTGGTAGGGGCGGTTTCAG(+CAGTGT)GATCCCACAGAAATGAAGGGACACAGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240605173420_56hm TGGGAGAAGACTGTGTCCCTTCATTTCTGTGG(+CAGTGT)GATCCTGAAACCGCCCCTACCAGGAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240605173418_aj5o CATCCAGGCCCCCAATCTTTTATATTGAAGGG(+CAGTGT)GATCCAACTTCCAGGATGAAAGGCAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605173417_0ui0 ACCAGGCTCTCTGCCTTTCATCCTGGAAGTTG(+CAGTGT)GATCCCCTTCAATATAAAAGATTGGGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605173415_2ovq GCTGAGCAAGAAAGGCTGTCTGAGCCATGGTG(+CAGTGT)GATCCCCAGGGAGGGAATGTCCCCTCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240605173413_r03x GGGCAGGGGGGAGGGGACATTCCCTCCCTGGG(+CAGTGT)GATCCACCATGGCTCAGACAGCCTTTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605173411_1hb6 GCCTACTTCTGGATCACCCTCATCCAGTGCTG(+CAGTGT)GTACCCCACAGCACTAAATCCAGGAACCCTG NGG 10 8 17 8 24 0 100 5 10
    240605173409_e0jm AAGCCCAGGGTTCCTGGATTTAGTGCTGTGGG(+CAGTGT)GTACCAGCACTGGATGAGGGTGATCCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605173408_0exn GGAAGCAGAAGGCAGAGCTGGCCCTGGTACTG(+CAGTGT)GTACCCCACCCTTCAGCGTAGCCCTTGGTGA NGG 10 8 17 8 24 0 100 5 10