Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173407_6qt6 AGCTGTCACCAAGGGCTACGCTGAAGGGTGGG(+CAGTGT)GTACCAGTACCAGGGCCAGCTCTGCCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605173405_8m6i AGAGAAAAGCAGACAGGCGGGGAACCTGCCAG(+CAGTGT)GTACCAGGGCGTTCCGCAGCGAATGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240605173402_o2qg CCCAGAATTCTCATTCGCTGCGGAACGCCCTG(+CAGTGT)GTACCTGGCAGGTTCCCCGCCTGTCTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605173400_gtms GAGAGAAACAGAGGGATAAAGAGGAGGCTCGG(+CAGTGT)GTACCACTATGGTTCCAGAGTCCTCTGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605173359_dk1z GGTAAAGGGCAGAGGACTCTGGAACCATAGTG(+CAGTGT)GTACCCGAGCCTCCTCTTTATCCCTCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605173357_wc34 GGTCACAATTGTCCCTAAATCTAGTTCCAAGG(+CAGTGT)GATCCAGCATCTTCTGGCCTCCGTGGGCACC NGG 10 8 17 8 24 0 100 5 10
    240605173355_pogb TGCCTGGTGCCCACGGAGGCCAGAAGATGCTG(+CAGTGT)GATCCCTTGGAACTAGATTTAGGGACAATTG NGG 10 8 17 8 24 0 100 5 10
    240605173354_ko78 CTTGGTTAAAATTAGTTCTCACTCCATGTATG(+CAGTGT)GATCCTTCAACTTCTCTTCAGAGAGAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605173353_i2j7 TGAGGACTTCTCTCTCTGAAGAGAAGTTGAAG(+CAGTGT)GATCCATACATGGAGTGAGAACTAATTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605173352_3mux TAAGTGAGACAAGAGCCCAGCCCAACCTCAGG(+CAGTGT)GATCCAACAGTCCACGGCCATCTCCTTAGCT NGG 10 8 17 8 24 0 100 5 10