Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605173438_9bdh GACCAGGAGAGGGTCCTGGAGTACCTTGGATG(+CAGTGT)GTACCCTCACTTCAAGCTCACCTCCCTCGGG NGG 10 8 17 8 24 0 100 5 10
    240605173436_sj7z GGACCGGCCTTTACTGAGCTGTCACACAGTGG(+CAGTGT)GTACCTCTAGGTGCTTTTGGGATGGCAGGTA NGG 10 8 17 8 24 0 100 5 10
    240605173435_69ai AGAGATACCTGCCATCCCAAAAGCACCTAGAG(+CAGTGT)GTACCCACTGTGTGACAGCTCAGTAAAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605173434_lxt3 CAGGGGATTTCATCAAGAATCCCAACTATTTG(+CAGTGT)GTACCAGAGCCAAGCAAATGCAAGCAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605173433_f8xh CCAAGTGTTCTGCTTGCATTTGCTTGGCTCTG(+CAGTGT)GTACCAAATAGTTGGGATTCTTGATGAAATC NGG 10 8 17 8 24 0 100 5 10
    240605173431_eer5 GAGCTGCTGCCACACTCGGGCCCAGCGAGGAG(+CAGTGT)GATCCGGGAAAGGCACCTCGGAACTCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605173429_4ubl CTTTGTGGCTGAGTTCCGAGGTGCCTTTCCCG(+CAGTGT)GATCCTCCTCGCTGGGCCCGAGTGTGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605173427_ybnv GTCTTCTCCCAATCACAGTTCCAGGGGCCAGG(+CAGTGT)GTACCTCCAGAGGACTTCTCAAGCTGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240605173426_v6n7 GAAACCCAACCAGCTTGAGAAGTCCTCTGGAG(+CAGTGT)GTACCCTGGCCCCTGGAACTGTGATTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605173424_cbl7 TATACTTGGGGACAGTTACAAGGGAGTCACAG(+CAGTGT)GTACCCTGTCCATGTCTGCGGCTGTGGGCAG NGG 10 8 17 8 24 0 100 5 10